Evaluation of the BDProbeTec ET system for direct detection of Mycobacterium tuberculosis in pulmonary and extrapulmonary samples:: a multicenter study

被引:28
|
作者
Mazzarelli, G
Rindi, L
Piccoli, P
Scarparo, C
Garzelli, C
Tortoli, E
机构
[1] Careggi Hosp, Reg Reference Ctr Mycobacteria, Microbiol & Virol Lab, Piastra Serv, I-50134 Florence, Italy
[2] Careggi Hosp, Microbiol & Virol Serum Immunol Lab, I-50134 Florence, Italy
[3] Univ Pisa, Dept Expt Pathol Med Biotechnol Infectivol & Epid, Pisa, Italy
[4] San Bortolo Hosp, Reg Reference Ctr Mycobacteria, Vicenza, Italy
关键词
D O I
10.1128/JCM.41.4.1779-1782.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We evaluated the BDProbeTec ET system (Becton Dickinson, Sparks, Md.), a strand displacement amplification-based technique, for direct detection of Mycobacterium tuberculosis in 867 clinical samples. Of 294 extrapulmonary specimens, 52 had positive results by both BDProbeTec ET and culture and 209 had negative results by both methods; sensitivity and specificity were 76.5 and 95.9%, respectively. After resolution of discrepancies, the sensitivity rose to 77.8%.
引用
收藏
页码:1779 / 1782
页数:4
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