Efficient gene editing in adult mouse livers via adenoviral delivery of CRISPR/Cas9

被引：82

作者：

Cheng, Ranran ^{[1
,2
]}

Peng, Jin ^{[1
,3
,4
]}

Yan, Yonghong ^{[1
]}

Cao, Peili ^{[1
]}

Wang, Jiewei ^{[1
,5
]}

Qiu, Chen ^{[1
]}

Tang, Lichun ^{[1
]}

Liu, Di ^{[1
]}

Tang, Li ^{[1
]}

Jin, Jianping ^{[6
]}

Huang, Xingxu ^{[7
]}

He, Fuchu ^{[1
,8
]}

Zhang, Pumin ^{[1
,8
,9
]}

机构：

[1] Beijing Inst Radiat Med, Beijing Prote Res Ctr, State Key Lab Prote, Beijing 100850, Peoples R China

[2] Beijing Univ Technol, Coll Life Sci & Bioengn, Beijing 100142, Peoples R China

[3] E China Normal Univ, Inst Biomed Sci, Shanghai 200241, Peoples R China

[4] E China Normal Univ, Sch Life Sci, Shanghai 200241, Peoples R China

[5] Zhejiang Univ, Coll Med, Affiliated Hosp 1, Dept Gastroenterol, Hangzhou 310011, Zhejiang, Peoples R China

[6] Univ Texas Hlth Sci Ctr Houston, Dept Biochem & Mol Biol, Houston, TX 77030 USA

[7] Nanjing Univ, Model Anim Res Ctr, Nanjing 210061, Jiangsu, Peoples R China

[8] Natl Ctr Prot Sci Beijing, Beijing 102206, Peoples R China

[9] Baylor Coll Med, Dept Mol Physiol & Biophys, Houston, TX 77030 USA

来源：

FEBS LETTERS | 2014年 / 588卷 / 21期

基金：

中国国家自然科学基金;

关键词：

CRISPR/Cas9; Gene editing; Liver; Adenoviruses; BINDING-PROTEIN-ALPHA; ONE-STEP GENERATION; CONDITIONAL ALLELES; MICE; HOMEOSTASIS; MUTATIONS; SYSTEMS; CAS9;

D O I：

10.1016/j.febslet.2014.09.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed an adenovirus-based CRISPR/Cas9 system for gene editing in vivo. In the liver, we demonstrated that the system could reach the level of tissue-specific gene knockout, resulting in phenotypic changes. Given the wide spectrum of cell types susceptible to adenoviral infection, and the fact that adenoviral genome rarely integrates into its host cell genome, we believe the adenovirus-based CRISPR/Cas9 system will find applications in a variety of experimental settings. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3954 / 3958

页数：5

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