Efficient gene editing in adult mouse livers via adenoviral delivery of CRISPR/Cas9

被引：82

作者：

Cheng, Ranran ^{[1
,2
]}

Peng, Jin ^{[1
,3
,4
]}

Yan, Yonghong ^{[1
]}

Cao, Peili ^{[1
]}

Wang, Jiewei ^{[1
,5
]}

Qiu, Chen ^{[1
]}

Tang, Lichun ^{[1
]}

Liu, Di ^{[1
]}

Tang, Li ^{[1
]}

Jin, Jianping ^{[6
]}

Huang, Xingxu ^{[7
]}

He, Fuchu ^{[1
,8
]}

Zhang, Pumin ^{[1
,8
,9
]}

机构：

[1] Beijing Inst Radiat Med, Beijing Prote Res Ctr, State Key Lab Prote, Beijing 100850, Peoples R China

[2] Beijing Univ Technol, Coll Life Sci & Bioengn, Beijing 100142, Peoples R China

[3] E China Normal Univ, Inst Biomed Sci, Shanghai 200241, Peoples R China

[4] E China Normal Univ, Sch Life Sci, Shanghai 200241, Peoples R China

[5] Zhejiang Univ, Coll Med, Affiliated Hosp 1, Dept Gastroenterol, Hangzhou 310011, Zhejiang, Peoples R China

[6] Univ Texas Hlth Sci Ctr Houston, Dept Biochem & Mol Biol, Houston, TX 77030 USA

[7] Nanjing Univ, Model Anim Res Ctr, Nanjing 210061, Jiangsu, Peoples R China

[8] Natl Ctr Prot Sci Beijing, Beijing 102206, Peoples R China

[9] Baylor Coll Med, Dept Mol Physiol & Biophys, Houston, TX 77030 USA

来源：

FEBS LETTERS | 2014年 / 588卷 / 21期

基金：

中国国家自然科学基金;

关键词：

CRISPR/Cas9; Gene editing; Liver; Adenoviruses; BINDING-PROTEIN-ALPHA; ONE-STEP GENERATION; CONDITIONAL ALLELES; MICE; HOMEOSTASIS; MUTATIONS; SYSTEMS; CAS9;

D O I：

10.1016/j.febslet.2014.09.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed an adenovirus-based CRISPR/Cas9 system for gene editing in vivo. In the liver, we demonstrated that the system could reach the level of tissue-specific gene knockout, resulting in phenotypic changes. Given the wide spectrum of cell types susceptible to adenoviral infection, and the fact that adenoviral genome rarely integrates into its host cell genome, we believe the adenovirus-based CRISPR/Cas9 system will find applications in a variety of experimental settings. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3954 / 3958

页数：5

共 50 条

[1] Efficient Editing of an Adenoviral Vector Genome with CRISPR/Cas9
Li, Qiang
Wang, Hui
Gong, Chen-yu
Chen, Zhao
Yang, Jia-xing
Shao, Hong-wei
Zhang, Wen-feng
INDIAN JOURNAL OF MICROBIOLOGY, 2021, 61 (01) : 91 - 95
[2] Efficient Editing of an Adenoviral Vector Genome with CRISPR/Cas9
Qiang Li
Hui Wang
Chen-yu Gong
Zhao Chen
Jia-xing Yang
Hong-wei Shao
Wen-feng Zhang
Indian Journal of Microbiology, 2021, 61 : 91 - 95
[3] CRISPR/CAS9 GENE EDITING
不详
CHEMICAL & ENGINEERING NEWS, 2015, : 26 - 27
[4] Delivery methods for CRISPR/Cas9 gene editing in crustaceans
Xu, Sen
Pham, Thinh
Neupane, Swatantra
MARINE LIFE SCIENCE & TECHNOLOGY, 2020, 2 (01) : 1 - 5
[5] Delivery methods for CRISPR/Cas9 gene editing in crustaceans
Sen Xu
Thinh Phu Pham
Swatantra Neupane
Marine Life Science & Technology, 2020, 2 : 1 - 5
[6] Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing
Zhang, Song
Shen, Jiangtao
Li, Dali
Cheng, Yiyun
THERANOSTICS, 2021, 11 (02): : 614 - 648
[7] Gene editing in mouse zygotes using the CRISPR/Cas9 system
Wefers, Benedikt
Bashir, Sanum
Rossius, Jana
Wurst, Wolfgang
Kuehn, Ralf
METHODS, 2017, 121 : 55 - 67
[8] Combining CRISPR/Cas9 and rAAV Templates for Efficient Gene Editing
Kaulich, Manuel
Dowdy, Steven F.
NUCLEIC ACID THERAPEUTICS, 2015, 25 (06) : 287 - 296
[9] Two efficient CRISPR/Cas9 systems for gene editing in soybean
Jéssica Carrijo
Eudald Illa-Berenguer
Peter LaFayette
Nathalia Torres
Francisco J. L. Aragão
Wayne Parrott
Giovanni R. Vianna
Transgenic Research, 2021, 30 : 239 - 249
[10] Efficient BoPDS Gene Editing in Cabbage by the CRISPR/Cas9 System
Ma, Cunfa
Liu, Mengci
Li, Qinfei
Si, Jun
Ren, Xuesong
Song, Hongyuan
HORTICULTURAL PLANT JOURNAL, 2019, 5 (04) : 164 - 169

← 1 2 3 4 5 →