Turning a Negative into a Positive: Conversion of a Homodimer into a Heterodimer Using Negative State Repertoires

被引：1
|
作者：
Davey, James A. [1 ]
Chica, Roberto A. [1 ]
机构：
[1] Univ Ottawa, Dept Chem & Biomol Sci, Ottawa, ON K1N 6N5, Canada
来源：
STRUCTURE | 2016年 / 24卷 / 04期
关键词：
PROTEIN DESIGN; ANTIBODY; SPECIFICITY; FC;
D O I：
10.1016/j.str.2016.03.012
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this issue of Structure, Leaver-Fay et al. (2016) engineer bispecific antibodies using multistate computational protein design with negative state repertoires. In combination with additional mutations selected rationally, they produced antibodies that assembled as heterodimers with up to 93% purity.
引用
收藏
页码：496 / 497
页数：2
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