Cell growth restoration and high level protein expression by the promoter of hexose transporter, HXT7, from Saccharomyces cerevisiae

被引：10
|
作者：
Lai, Ming-Tsong
Liu, Daniel Yuen-Teh
Hseu, Tzong-Hsiung
机构：
[1] Natl Tsing Hua Univ, Inst Biotechnol, Hsinchu 30013, Taiwan
[2] Natl Tsing Hua Univ, Dept Life Sci, Hsinchu 30013, Taiwan
来源：
BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 08期
关键词：
hexose transporter; invertase; protein expression; Saccharomyces cerevisiae;
D O I：
10.1007/s10529-007-9397-3
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The promoters of high-affinity hexose transporter, HXT6 and HXT7, are sufficient for complementary expression of invertase to restore the growth of Saccharomyces cerevisiae in raffinose medium. The HXT7 promoter produced higher invertase activity at 139- and 30-fold of the basal activity in strains GN 3C.2 and W303-1, respectively. In addition, the HXT7 promoter expressed 3- to 9-fold more of enhanced green fluorescent protein than that of the constitutive ADH1 in three different S. cerevisiae strains, even during short-term incubation in glucose medium. Therefore, HXT7 promoter could be used for heterologous protein expression in S. cerevisiae.
引用
收藏
页码：1287 / 1292
页数：6
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