Intramolecular complementation of measles virus fusion protein stability confers cell-cell fusion activity at 37 °C

The fusion (F) protein of measles virus mediates membrane fusion. In this study, we investigated the molecular basis of the cell-cell fusion activity of the F protein. The N465H substitution in the heptad repeat B domain of the stalk region of the F protein eliminates this activity, but an additional mutation in the DIII domain of the head region - N183D, F217L, P219S, I225T or G240R - restores cell-cell fusion. Thermodynamically stabilized by the N465H substitution, the F protein required elevated temperature as high as 40 degrees C to promote cell-cell fusion, whereas all five DIII mutations caused destabilization of the F protein allowing the highest fusion activity at 30 degrees C. Stability complementation between the two domains conferred an efficient cell-cell fusion activity on the F protein at 37 degrees C. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.