Higenamine mitigates interleukin-1β-induced human nucleus pulposus cell apoptosis by ROS-mediated PI3K/Akt signaling

被引:20
|
作者
Zhu, Xiaojuan [1 ]
Liu, Shichao [2 ]
Cao, Zhijiao [1 ]
Yang, Lei [3 ]
Lu, Fang [4 ]
Li, Yulan [1 ]
Hu, Lili [5 ]
Bai, Xiaoliang [4 ]
机构
[1] Baoding 1 Cent Hosp, Dept Geriatr, Baoding 071000, Peoples R China
[2] Baoding 1 Cent Hosp, Emergency Dept, Baoding 071000, Peoples R China
[3] Baoding 1 Cent Hosp, Dept Anesthesiol, Baoding 071000, Peoples R China
[4] Baoding 1 Cent Hosp, Dept Orthoped, 320 North Changcheng Ave, Baoding 071000, Peoples R China
[5] Laishui Cty Hosp, Dept Resp Med, Baoding 071000, Peoples R China
关键词
Intervertebral disc degeneration; Higenamine; Apoptosis; ROS; PI3K; Akt pathway; INTERVERTEBRAL DISC DEGENERATION; OXIDATIVE STRESS; HEME OXYGENASE-1; INFLAMMATION; ACTIVATION; INDUCTION; INJURY;
D O I
10.1007/s11010-021-04197-z
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Intervertebral disc degeneration (IDD) is a natural problem linked to the inflammation. Higenamine exerts multiple pharmacological properties in inflammation-related disorders. Our study aimed to explore the function of higenamine on interleukin (IL)-1 beta-caused apoptosis of human nucleus pulposus cells (HNPCs). Cell apoptosis was investigated by TUNEL and flow cytometry. Apoptosis-related biomarkers were determined by qRT-PCR or Western blotting. The protein in the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling was measured by Western blotting. We found that higenamine showed little effect on cell apoptosis, but mitigated IL-1 beta-caused apoptosis in a dose-dependent pattern. Higenamine attenuated IL-1 beta-induced decrease of Bcl-2 and increase of Bax and cleaved caspase-3. Higenamine did not affect the reactive oxygen species (ROS) level and the PI3K/Akt signaling, but attenuated IL-1 beta-induced ROS production and inhibition of the PI3K/Akt signaling. IL-1 beta repressed the activation of the PI3K/Akt pathway, but ROS inhibition using N-acetylcysteine (NAC) rescued this pathway. The PI3K/Akt signaling suppression using LY294002 reversed the inhibitive effect of higenamine on IL-1 beta-caused apoptosis, and this effect was weakened by ROS inhibition. In conclusion, higenamine attenuates IL-1 beta-caused apoptosis of HNPCs via ROS-mediated PI3K/Akt pathway.
引用
收藏
页码:3889 / 3897
页数:9
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