Covalent immobilization of oligonucleotides on modified glass/silicon surfaces for solid-phase DNA hybridization and amplification

被引：28
|
作者：
Yang, MS [1 ]
Kong, RYC [1 ]
Kazmi, N [1 ]
Leung, AKC [1 ]
机构：
[1] City Univ Hong Kong, Dept Biol & Chem, Kowloon, Hong Kong
来源：
CHEMISTRY LETTERS | 1998年 / 03期
关键词：
D O I：
10.1246/cl.1998.257
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Glass slides and silicon chips were modified with aminosilane and various linker molecules to provide activated surfaces for covalent immobilization of oligonucleotides under ambient condition, and the surface-attached oligonucleotides remain active for solid-phase DNA hybridization and polymerase chain reaction.
引用
收藏
页码：257 / 258
页数：2
相关论文
共 50 条
  • [21] Phosphoramidite Reagents and Solid-Phase Supports Based on Hydroxyprolinol for the Synthesis of Modified Oligonucleotides
    Tatulchenkov, M. Yu.
    Prokhorenko, I. A.
    Kvach, M. V.
    Navakouski, M. E.
    Stepanova, I. A.
    Pilchenko, N. V.
    Gontarev, S. V.
    Sharko, O. L.
    Korshun, V. A.
    Shmanai, V. V.
    RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY, 2017, 43 (04) : 386 - 396
  • [22] DNA-Modified Electrodes (IX) - Immobilization and Characterization of DNA Probes and the Molecular Hybridization on Gold Surfaces
    Zhao, Yuan-Di
    Pang, Dai-Wen
    Zhang, Min
    Cheng, Jie-Ke
    Dai, Hong-Ping
    Kao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal of Chinese Universities, 2001, 22 (05):
  • [23] DNA-modified electrodes (IX) - Immobilization and characterization of DNA probes and the molecular hybridization on gold surfaces
    Zhao, YD
    Pang, DW
    Zhang, M
    Cheng, JK
    Dai, HP
    CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE, 2001, 22 (05): : 744 - 748
  • [24] Parameters controlling the one-step derivatization of controlled pore glass with a diol for solid-phase synthesis of 3′-modified oligonucleotides
    Laurent, A
    Chaix, C
    ORGANIC PROCESS RESEARCH & DEVELOPMENT, 2006, 10 (03) : 403 - 408
  • [25] Covalent end-immobilization of DNA strands onto solid surfaces and their subsequent functionalization.
    Laibinis, PE
    Lee, IH
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2001, 222 : U378 - U378
  • [26] SOLID-PHASE DISULFIDE OXIDES - A NEW APPROACH TO REVERSIBLE IMMOBILIZATION AND COVALENT CHROMATOGRAPHY OF THIOL COMPOUNDS
    CARLSSON, J
    BATISTAVIERA, F
    BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 1991, 14 (01) : 114 - 120
  • [27] COVALINK NH MICROWELL PLATES USED AS SOLID-PHASE FOR DNA HYBRIDIZATION ASSAYS
    RASMUSSEN, SR
    LARSEN, MR
    RASMUSSEN, SE
    MOLECULAR BIOLOGY OF THE CELL, 1992, 3 : A89 - A89
  • [28] Rotating rod renewable microcolumns for automated, solid-phase DNA hybridization studies
    Bruckner-Lea, CJ
    Stottlemyre, MS
    Holman, DA
    Grate, JW
    Brockman, FJ
    Chandler, DP
    ANALYTICAL CHEMISTRY, 2000, 72 (17) : 4135 - 4141
  • [29] Covalent immobilization of oligonucleotides on p-aminophenyl-modified carbon screen-printed electrodes for viral DNA sensing
    Ruffien, A
    Dequaire, M
    Brossier, P
    CHEMICAL COMMUNICATIONS, 2003, (07) : 912 - 913
  • [30] SOLID-PHASE EPITAXIAL SEED FOR LASER-CRYSTALLIZED SILICON ON GLASS SUBSTRATES
    DOI, A
    KUMIKAWA, M
    KONISHI, J
    NAKAMIZO, Y
    APPLIED PHYSICS LETTERS, 1991, 59 (20) : 2518 - 2520
12345