Long-lifetime lipid probe containing a luminescent metal-ligand complex

被引：0

作者：

Li, L ^{[1
]}

Szmacinski, H ^{[1
]}

Lakowicz, JR ^{[1
]}

机构：

[1] UNIV MARYLAND,SCH MED,CTR FLUORESCENCE SPECT,DEPT BIOCHEM & MOL BIOL,BALTIMORE,MD 21201

来源：

BIOSPECTROSCOPY | 1997年 / 3卷 / 02期

关键词：

fluorescence; metal-ligand probes; long lifetime probes; polarization; anisotropy; lipids; membranes;

D O I：

10.1002/(SICI)1520-6343(1997)3:2<155::AID-BSPY8>3.0.CO;2-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the chemical synthesis and spectral properties of a long-lifetime luminescent probe for membranes. A ruthenium metal-ligand complex was covalently coupled to the amino group of phosphatidyl ethanolamine. When incorporated into model membranes, this probe displays decay times near 500 ns. Importantly, the probe displays polarized emission and can be used to study membrane motions on the microsecond timescale. (C) 1997 John Wiley & Sons, Inc.

引用

页码：155 / 159

页数：5

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