A sensitive assay for ZYAN1 in human whole blood and urine utilizing positive LC-MS/MS electrospray ionization

被引：1

作者：

Patel, Harilal ^{[1
]}

Soni, Krunal ^{[1
]}

Trivedi, Rucha ^{[1
]}

Heading, Heather ^{[2
]}

Geue, Jason ^{[2
]}

Kansagra, Kevinkumar ^{[3
]}

Gupta, Rahul J. ^{[3
]}

Pandya, Vrajesh B. ^{[4
]}

Srinivas, Nuggehally R. ^{[1
]}

Patel, Pankaj R. ^{[1
,3
,4
]}

Desai, Ranjit C. ^{[4
]}

机构：

[1] Zydus Res Ctr, Bioanalyt Lab, Ahmadabad 382210, Gujarat, India

[2] CPR Pharma Serv, Thebarton, SA 5031, Australia

[3] Zydus Res Ctr, Clin Res, Ahmadabad 382210, Gujarat, India

[4] Zydus Res Ctr, Med Chem, Ahmadabad 382210, Gujarat, India

来源：

BIOANALYSIS | 2017年 / 9卷 / 09期

关键词：

anemia; LC-MS/MS; method validation; pharmacokinetics; prolyl hydroxylase inhibitors; whole blood; ZYAN1; PROLYL HYDROXYLASE INHIBITOR; ROXADUSTAT FG-4592; ANEMIA; HYPOXIA; DISEASE; ALPHA;

D O I：

10.4155/bio-2017-0014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aim: A sensitive LC-MS/MS method was developed and validated for estimation of ZYAN1 in human blood/urine. Methods: An analog internal standard IOX2 along with ZYAN1 was quantified using selective reaction monitoring in positive mode. The chromatographic separation was performed by gradient elution with C-18 analytical column (3 mu m, 50 mm x 2.0 mm) with 4-min run time using an acidified mobile phase consisting of ammonium formate and acetonitrile. Protein precipitation enabled extraction of analytes from diluted blood/urine. Results: Calibration curve of ZYAN1 was linear (2-5000 ng/ml). The recovery of ZYAN1 and IOX2 was between 87 and 104%. Interday and intraday accuracy and precision was found well within the acceptance criteria. Conclusion: The validated assay was applied for clinical pharmacokinetics of ZYAN1 in healthy volunteers.

引用

页码：719 / 732

页数：14

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