Genetic diversity and its implications in the conservation of endangered Zostera japonica in Korea

As part of the on-going effort to conserve endangered Zostera japonica Ascher. & Graebn. in Korea, we have used RAPID band patterns to analyze its genetic structure and diversity. Out of 50 primers tested, 45 formed amplified bands with its genome, including 814 polymorphic and 28 monomorphic bands. The highest number 0 20) was found in the population of Geoje-do; the smallest (58), in Anmyeon-do. An examination of its genetic structure with AMOVA revealed that about 50% of all variations could equally be assigned to within and between populations. The statistical value G(ST) (index of genetic differences) was 0.49, and the average number of individuals exchanged between populations per generation (N(e)m) was calculated as 0.26. Although the habitats of Z. japonica in Korea are disappearing at an alarming rate, significant levels of genetic variation still exist, especially in the Geoje-do population. Therefore, any recovery strategy for this endangered species should be planned on the basis of this genetic diversity among populations.