Vital Lipids Contents in Buffalo Butter Oil and its Fractions Prepared by Dry Fractionation

Background and Objective: The fractionation of butter oil to various specific fractions needs recently to multiply its uses and the requirement of its interchangeability. This article is concerning with the distribution of bioactive lipids in buffalo butter-oil and its fractions prepared by the dry fractionation technique. Materials and Methods: Thirty-two samples of Egyptian fresh buffalo milk were collected, divided into 4 composite samples and then converted to butter oil. Each butter oil sample was fractionated to four fractions: three solid fraction treatments at 35 degrees C (S-35), 25 degrees C (S-25), 15 degrees C (S-15)and liquid fraction at 15 degrees C (L15). Fatty acids profile was assayed included butyric acid (BA), short chain fatty acids (SCFAs), conjugated linoleic acid (CLA), odd & branched chain fatty acids (OBCFAs) and Trans fatty acids by using GC-MS apparatus. Results: Dry fractionation of butter-oil revealed that L15 had a higher content of CLA as well conjugated diene and triene fatty acids, while S25 contained higher content of total OBCFAs as compared to all other fractions. Conclusion: The dry fractionation leads to marked differences in the distribution of bioactive lipids in buffalo butter oil and its fractions. Each fraction could be used for specific purposes in the food industries according to its melting properties and this affect its healthy benefit.