Cloning, characterization and expression of a new cry1Ab gene from Bacillus thuringiensis WB9

被引：8

作者：

Huang, ZP ^{[1
]}

Guan, CH

Guan, X

机构：

[1] Fujian Agr & Forestry Univ, Minist Educ, Key Lab Biopesticide & Chem Biol, Fujian 350002, Peoples R China

[2] Fujian Agr & Forestry Univ, Minist Educ, Coll Life Sci, Ctr Biotechnol, Fujian 350002, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 20期

关键词：

Bacillus thuringiensis; bioassay; cloning and expression; cry1Ab17; gene; sequence analysis;

D O I：

10.1023/B:BILE.0000045652.00137.1f

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new cry1Ab-type gene, cry1Ab17, was cloned from Bacillus thuringiensis WB9 by PCR. Nucleotide sequence indicated that the open reading frames (ORFs) consists of 3471 bases and encodes a protein of 1156 residues with a calculated molecular weight of 130.5 kDa and an pI value of 5.04. Homology comparison revealed that the deduced amino acid sequence of Cry1Ab17 had 95.4% to 99.7% identity with those of the known Cry1Ab proteins. The Cry1Abl7 was one residue longer than the known Cry1Ab (except for Cry1Ab2). Domain I (Tyr(33) to Arg(253)), II (Arg(265) to Phe(462)), III (Asn(464) to Thr(610)) of the Cry1Ab17 were 96.8%, 68.2% and 100% identical to the corresponding domains of Cry1Aa. Additionally, the cry1Ab17 gene was expressed in Escherichia coli BL21 under the control of T7 promoter and the Cry1Ab17 isolated from the culture medium was toxic to 3rd instar Plutella xylostella larvae.

引用

页码：1557 / 1561

页数：5

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