A two-step chondrocyte recovery system based on thermally sensitive elastin-like polypeptide scaffolds for cartilage tissue engineering

被引：0

作者：

Betre, H ^{[1
]}

Chilkoti, A ^{[1
]}

Setton, LA ^{[1
]}

机构：

[1] Duke Univ, Dept Biomed Engn, Durham, NC 27706 USA

来源：

SECOND JOINT EMBS-BMES CONFERENCE 2002, VOLS 1-3, CONFERENCE PROCEEDINGS: BIOENGINEERING - INTEGRATIVE METHODOLOGIES, NEW TECHNOLOGIES | 2002年

关键词：

cartilage; scaffold; elastin; chondrocyte; tissue engineering;

D O I：

暂无

中图分类号：

TP18 [人工智能理论];

学科分类号：

081104 ; 0812 ; 0835 ; 1405 ;

摘要：

A "two step" tissue engineering strategy was developed to promote rapid matrix accumulation in cartilage constructs in vitro. Chondrocytes expanded in monolayer were encapsulated and cultured in a genetically engineered, thermally sensitive elastin-like polypeptide (ELP) for ten days. The resulting cell-matrix pellets were recovered from the ELP and cultured on inserts for up to four weeks, where nutrient diffusion was not impeded by the presence of the scaffold. Approximately two-milligram (dry weight) tissue was generated that resembles native articular cartilage in histological appearance and biochemical composition. These results suggest that rapid and large cartilage construct formation is possible in vitro, following a period of early incubation and recovery from the thermally responsive ELP.

引用

页码：829 / 830

页数：2