Inhibitory effect of amphotericin B on leukotriene B4 synthesis in human neutrophils in vitro

被引：4

作者：

Shindo, K ^{[1
]}

Fukumura, M ^{[1
]}

Ito, A ^{[1
]}

机构：

[1] Yokohama City Univ, Sch Med, Dept Internal Med 1, Kanazawa Ku, Yokohama, Kanagawa 236, Japan

来源：

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 1998年 / 58卷 / 02期

关键词：

D O I：

10.1016/S0952-3278(98)90148-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Our objective was to determine the effect of amphotericin B on leukotriene (LT) A(4) hydrolase in human neutrophil cytosol and to examine its effect on intact neutrophils in vitro. Cytosolic fractions were assayed for LTA(4) hydrolase and 5-lipoxygenase activity in the presence or absence of amphotericin B. The IC50 of amphotericin B for LTA(4) hydrolase activity was 0.72 mu M. No inhibition of 5-lipoxygenase activity in the cytosolic fraction was detected. The IC50 of amphotericin B for leukotriene B-4 synthesis in intact neutrophils was 0.43 mu M. The 5-hydro(per)oxy-eicosatetraenoic acid (5-H(P)ETE) synthesis was diminished in intact cells by 66.8 [3.4]% (mean[SEM]) in the presence of 0.01 mM amphotericin B. Thus, amphotericin B inhibited the synthesis of LTB4 and 5-H(P)ETE in neutrophils in vitro. Differences between the results of studies on cytosol and on intact cells suggest that amphotericin B is involved in a complex interaction in the intact cell.

引用

页码：105 / 109

页数：5

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