Synthesis, Dynamic Combinatorial Chemistry, and PCR Amplification of 3′-5′ and 3′-6′ Disulfide-linked Oligonucleotides

被引：17

作者：

Hansen, Dennis Jul ^{[1
,2
]}

Manuguerra, Ilenia ^{[1
,2
]}

Kjelstrup, Michael Brondum ^{[1
,2
]}

Gothelf, Kurt Vesterager ^{[1
,2
]}

机构：

[1] Danish Natl Res Fdn, Ctr DNA Nanotechnol, Dept Chem, DK-8000 Aarhus C, Denmark

[2] iNANO, DK-8000 Aarhus C, Denmark

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2014年 / 53卷 / 52期

基金：

新加坡国家研究基金会;

关键词：

disulfides; dynamic combinatorial chemistry; nucleic acids; primer extension; solid-phase synthesis; ION-PROMOTED UNBLOCKING; PHAGE DISPLAY; MACROCYCLIC DISULFIDES; DNA; LIBRARIES; SELECTION; LINKAGE;

D O I：

10.1002/anie.201405761

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Disulfide dithymidines linked 3'-5' or 3'-6' were synthesized and incorporated into oligonucleotides through a combined phosphotriester and phosphoramidite solid-phase oligonucleotide synthesis approach. The disulfide links are cleaved and formed reversibly in the presence of thiols and oligonucleotides. This link was shown to be sequence-adaptive in response to given templates in the presence of mercaptoethanol. The artificial 3'-5' and 3'-6' disulfide link was tolerated by polymerases in the polymerase chain reaction (PCR). By using sequencing analysis, we show that single mutations frequently occurred randomly in the amplification products of the PCR.

引用

页码：14415 / 14418

页数：4

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