A microplate-based method for real-time monitoring of Saccharomyces cerevisiae viability

被引:1
|
作者
Ye, Yan-Rui [1 ]
Ye, Mei-Li [1 ]
Li, Li-Li [1 ]
Lin, Ying [1 ]
机构
[1] S China Univ Technol, Sch Biosci & Bioengn, Guangzhou, Guangdong, Peoples R China
关键词
YEAST VIABILITY; FLOW-CYTOMETRY; ASSAYS;
D O I
10.1016/j.ab.2010.05.009
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Measuring the viability of the cells after chemical or physical stresses is a routine method in the determination of phenotypes in yeast. Numerous analytical techniques have been applied to the measurement of yeast cell viability, but few of them are suitable for real-time monitoring. Here we propose a microplate-based method for real-time monitoring of Saccharomyces cerevisiae cell viability. This method is also suitable for high-throughput measurement. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码:144 / 146
页数:3
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