A pH-sensitive red fluorescent protein compatible with hydrophobic

被引：0
|
作者：
Guo, Wenyan [1 ,2 ]
Gang, Yadong [1 ,2 ]
Liu, Xiuli [1 ,2 ]
Zhou, Hongfu
Zeng, Shaoqun [1 ,2 ]
机构：
[1] Huazhong Univ Sci & Technol, Britton Chance Ctr Biomed Photon, Wuhan Natl Lab Optoelect, 1037 Luoyu Rd, Wuhan 430074, Hubei, Peoples R China
[2] Huazhong Univ Sci & Technol, Dept Biomed Engn, Key Lab Biomed Photon, Minist Educ, 1037 Luoyu Rd, Wuhan 430074, Hubei, Peoples R China
来源：
NEURAL IMAGING AND SENSING | 2017年 / 10051卷
基金：
国家高技术研究发展计划(863计划);
关键词：
pH-sensitive; red fluorescent protein; hydrophobic resin; chemical reactivation; pHuji; ORANGE; TOMOGRAPHY; RESOLUTION; BRAIN;
D O I：
10.1117/12.2250844
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
pH sensitive fluorescent proteins enabling chemical reactivation in resin are useful tools for fluorescence microimaging. EYFP or EGFP improved from GFP in jellyfish are good for such applications. For simultaneous two-color imaging, a suitable red fluorescent protein is of urgent need. Here a pH sensitive red fluorescent protein, pHuji, is selected and verified to be compatible with hydrophobic resin embedding and thus may be promising for dual-colour chemical reactivation imaging in conjunction with EGFP or EYFP.
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页数：4
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