Two-photon Fluorescent Labeling Kit for the Real-time Dynamic Biopsy of CA19-9

被引：3

作者：

Huang Chibao ^{[1
,2
]}

Zhang Daohai ^{[3
]}

Zeng Boping ^{[1
]}

Liu Qibin ^{[3
]}

Chen Huashi ^{[1
]}

Kang Shuai ^{[1
]}

Chen Xiaoyuan ^{[2
]}

机构：

[1] Zunyi Normal Univ, Chem & Chem Engn Coll, Zunyi 563002, Peoples R China

[2] Shaoguan Univ, Coll Agr Sci & Engn, Shaoguan 512005, Peoples R China

[3] Guizhou Univ, Coll Mat & Met, Guiyang 550025, Peoples R China

来源：

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2016年 / 37卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Carbonhydrate antigen CA19-9; Antibody Ab19-9; Immunofluorescence labeling; Two-photon; Biopsy; CROSS-SECTIONS; ONE-PHOTON; FLUOROPHORES; CARCINOMA; SERIES; PROBE; IONS;

D O I：

10.7503/cjcu20150727

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Carbohydrate antigen CA19-9(Ag) is a tumor marker associated with pancreatic cancer, gallbladder cancer, colon cancer and gastric cancer in early stage of tumor and cancer stage. Antibody Ab19-9(Ab) to carbohydrate antigens were labeled to form probe. labeled antibody LP-Ab using the prepared two. photon fluorescence probe LP. On the basis of the antigen. antibody specificity affinity principle, LP-Ab could combine with carbohydrate antigen Ag into a complex LP-Ab . Ag, which was excited by near infrared laser(740 nm) and emitted strong two. photon fluorescence to accomplish the high sensitive biopsy and real. time dynamic high-resolution imaging of the tumor marker Ag with two. photon fluorescence microscope. Based on this, a simple and convenient two-photon fluorescent labeling kit was prepared to realize the rapid detection of tumor, which is convenient for large-scale clinic testing and screening. The research further broadens the application of LP, and provides a sharp tool for revealing the mechanism of cancer occurrence, development and metastasis. LP-Ab can be used to observe and study the dynamic transfer mechanisms of the tumor markers in cells and the mechanism of carcinogenesis.

引用

页码：638 / 642

页数：5

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