Two-photon Fluorescent Labeling Kit for the Real-time Dynamic Biopsy of CA19-9

Carbohydrate antigen CA19-9(Ag) is a tumor marker associated with pancreatic cancer, gallbladder cancer, colon cancer and gastric cancer in early stage of tumor and cancer stage. Antibody Ab19-9(Ab) to carbohydrate antigens were labeled to form probe. labeled antibody LP-Ab using the prepared two. photon fluorescence probe LP. On the basis of the antigen. antibody specificity affinity principle, LP-Ab could combine with carbohydrate antigen Ag into a complex LP-Ab . Ag, which was excited by near infrared laser(740 nm) and emitted strong two. photon fluorescence to accomplish the high sensitive biopsy and real. time dynamic high-resolution imaging of the tumor marker Ag with two. photon fluorescence microscope. Based on this, a simple and convenient two-photon fluorescent labeling kit was prepared to realize the rapid detection of tumor, which is convenient for large-scale clinic testing and screening. The research further broadens the application of LP, and provides a sharp tool for revealing the mechanism of cancer occurrence, development and metastasis. LP-Ab can be used to observe and study the dynamic transfer mechanisms of the tumor markers in cells and the mechanism of carcinogenesis.