Two potato proteins, including a novel RING finger protein (HIP1), interact with the potyviral multifunctional protein HCpro

被引:41
|
作者
Guo, DY
Spetz, C
Saarma, M
Valkonen, JPT
机构
[1] Univ Helsinki, Inst Biotechnol, Viikki Bioctr, FIN-00014 Helsinki, Finland
[2] SLU, Genet Ctr, Dept Plant Biol, SE-75007 Uppsala, Sweden
[3] Univ Helsinki, Dept Appl Biol, FIN-00014 Helsinki, Finland
[4] Wuhan Univ, Coll Life Sci, Virol Ctr, Wuhan 430072, Peoples R China
关键词
D O I
10.1094/MPMI.2003.16.5.405
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Potyviral helper-component proteinase (HCpro) is a multifunctional protein exerting its cellular functions in interaction with putative host proteins. In this study, cellular protein partners of the HCpro encoded by Potato virus A (PVA) (genus Potyvirus) were screened in a potato leaf cDNA library using a yeast two-hybrid system. Two cellular proteins were obtained that interact specifically with PVA HCpro in yeast and in the two in vitro binding assays used. Both proteins are encoded by single-copy genes in the potato genome. Analysis of the deduced amino acid sequences revealed that one (HIP1) of the two HCpro interactors is a novel RING finger protein. The sequence of the other protein (HIP2) showed no resemblance to the protein sequences available from databanks and has known biological functions.
引用
收藏
页码:405 / 410
页数:6
相关论文
共 50 条
  • [31] Isolation of S-RNase binding proteins from Solanum chacoense:: identification of an SBP1 (RING finger protein) orthologue
    O'Brien, M
    Major, G
    Chantha, SC
    Matton, DP
    SEXUAL PLANT REPRODUCTION, 2004, 17 (02): : 81 - 87
  • [32] Isolation of S-RNase binding proteins from Solanum chacoense: identification of an SBP1 (RING finger protein) orthologue
    Martin O’Brien
    Geneviève Major
    Sier-Ching Chantha
    Daniel P. Matton
    Sexual Plant Reproduction, 2004, 17 : 81 - 87
  • [33] Characterization and Potential Function of a Novel Pre-Implantation Embryo-Specific RING Finger Protein: TRIML1
    Tian, Liyuan
    Wu, Xiaojie
    Lin, Yanli
    Liu, Zhuguo
    Xiong, Fuyin
    Han, Zhengbin
    Zhou, Yanrong
    Zeng, Qiangcheng
    Wang, Yumin
    Deng, Jixian
    Chen, Hongxing
    MOLECULAR REPRODUCTION AND DEVELOPMENT, 2009, 76 (07) : 656 - 664
  • [34] RBBP6 Interacts with Multifunctional Protein YB-1 through Its RING Finger Domain, Leading to Ubiquitination and Proteosomal Degradation of YB-1
    Chibi, Moredreck
    Meyer, Mervin
    Skepu, Amanda
    Rees, D. Jasper G.
    Moolman-Smook, Johanna C.
    Pugh, David J. R.
    JOURNAL OF MOLECULAR BIOLOGY, 2008, 384 (04) : 908 - 916
  • [35] Novel RING finger protein OIP1 binds to conserved amino acid repeats in sperm tail protein ODF1 (vol 68, pg 543, 2002)
    Zarsky, HA
    Cheng, M
    van der Hoorn, FA
    BIOLOGY OF REPRODUCTION, 2003, 69 (04) : 1447 - 1447
  • [36] Novel RING finger proteins, Air1p and Air2p, interact with Hmt1p and inhibit the arginine methylation of Npl3p
    Inoue, K
    Mizuno, T
    Wada, K
    Hagiwara, M
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (42) : 32793 - 32799
  • [37] Rma1, a novel type of RING finger protein conserved from Arabidopsis to human, is a membrane-bound ubiquitin ligase
    Matsuda, N
    Suzuki, T
    Tanaka, K
    Nakano, A
    JOURNAL OF CELL SCIENCE, 2001, 114 (10) : 1949 - 1957
  • [38] Mrz1, a Novel Mitochondrial Outer Membrane RING Finger Protein, is Degraded Through the Ubiquitin–Proteasome Pathway in Schizosaccharomyces pombe
    Zecheng Liu
    Pan Zhang
    Minjie Li
    Limayan A
    Guihong Yang
    Yao Yu
    Hong Lu
    Jinjie Shang
    Ying Huang
    Current Microbiology, 2022, 79
  • [39] Rma1, a novel type of RING finger protein conserved from Arabidopsis to human, is a membrane-bound ubiquitin ligase
    Matsuda, N
    Suzuki, T
    Tanaka, K
    Nakano, A
    MOLECULAR BIOLOGY OF THE CELL, 2001, 12 : 68A - 68A
  • [40] ZFPL1, a novel ring finger protein required for cis-Golgi integrity and efficient ER-to-Golgi transport
    Chiu, Chi-Fang
    Ghanekar, Yashoda
    Frost, Laura
    Diao, Aipo
    Morrison, Daniel
    McKenzie, Eddie
    Lowe, Martin
    EMBO JOURNAL, 2008, 27 (07): : 934 - 947