Reverse transcriptase polymerase chain reaction for amantadine susceptibility testing of influenza viruses

被引：0
|
作者：
Suen, PC
Tsao, KC
Wu, TL
Ning, HC
Liu, TZ
Shih, SR
机构：
[1] Chang Gung Univ, Sch Med Technol, Tao Yuan 333, Taiwan
[2] Chang Gung Mem Hosp, Dept Dent, Tao Yuan, Taiwan
[3] Chang Gung Univ, Dept Physiol, Tao Yuan, Taiwan
[4] Chang Gung Mem Hosp, Dept Clin Pathol, Tao Yuan, Taiwan
[5] Yuans Gen Hosp, Clin Labs, Kaohsiung, Taiwan
[6] Yuans Gen Hosp, Dept Med Res, Kaohsiung, Taiwan
来源：
BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 12期
关键词：
D O I：
10.1023/A:1018454031252
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The clinical use of amantadine for influenza A viruses infection is increasing. A novel quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay was developed to determine the IC50 (dose of 50% inhibitory) of amantadine. The traditional amantadine susceptibility testing by plaque inhibition assay took 3 days to observe plaque formation. In contrast, the proposed assay took 3 hours to detect viral RNA. The RT-PCR products can be directly sequenced for screening and identifying the resistant strains.
引用
收藏
页码：873 / 878
页数：6
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