X and Y chromosome-bearing spermatozoa are equally able to uptake and internalize exogenous DNA by sperm-mediated gene transfer in swine

被引：2

作者：

Zaniboni, Andrea ^{[1
]}

Spinaci, Marcella ^{[1
]}

Zannoni, Augusta ^{[1
]}

Bernardini, Chiara ^{[1
]}

Forni, Monica ^{[1
]}

Bacci, Maria Laura ^{[1
]}

机构：

[1] Dept Vet Med Sci DIMEVET, Via Tolara 50, I-40064 Bologna, Italy

来源：

RESEARCH IN VETERINARY SCIENCE | 2016年 / 104卷

关键词：

Swine; Sperm sorting; SMGT; X/Y chromosome-bearing spermatozoa; Differences; SOMATIC-CELL CLONING; IN-VITRO; PROTEINS; LOCALIZATION; EXPRESSION; MEMBRANE; BOVINE; PIGS;

D O I：

10.1016/j.rvsc.2015.11.001

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Since proteomic differences between male X/Y chromosome-bearing gametes have recently been described, a question has been raised: could these differences be responsible for different behavior between X and Y chromosome-bearing spermatozoa during the binding and internalization of exogenous DNA in the swine species? In order to investigate this hypothesis, our group studied the process of the uptake and internalization of exogenous DNA in X and Y chromosome-bearing sperm sub-populations. No significant differences were found between sperm types in both the uptake and internalization of exogenous DNA. The quantity of internalized exogenous DNA was significantly lower than that of the uptaken DNA. In conclusion, our results showed that X and Y chromosomes-bearing spermatozoa have the same binding capacity and internalization of DNA, and the proteomic differences between them do not seem to interfere with these complex processes. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：1 / 3

页数：3