Rapid Identification and Differentiation of Mycobacterium avium Subspecies paratuberculosis Types by Use of Real-Time PCR and High-Resolution Melt Analysis of the MAP1506 Locus

被引：11
|
作者：
Castellanos, Elena [2 ,3 ]
Aranaz, Alicia [2 ,3 ]
De Buck, Jeroen [1 ]
机构：
[1] Univ Calgary, Dept Prod Anim Hlth, Fac Vet Med, Calgary, AB T2N 4N1, Canada
[2] Univ Complutense Madrid, Ctr Vigilancia Sanitaria Vet, VISAVET, E-28040 Madrid, Spain
[3] Univ Complutense Madrid, Fac Vet, Dept Sanidad Anim, E-28040 Madrid, Spain
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 04期
基金：
加拿大自然科学与工程研究理事会;
关键词：
RESTRICTION ENDONUCLEASE ANALYSIS; FIELD GEL-ELECTROPHORESIS; DNA HYBRIDIZATION; POLYMORPHISMS; STRAINS; SEQUENCE; COMPLEX; GENES; GOATS;
D O I：
10.1128/JCM.02484-09
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on type-specific nucleotide sequences in MAP1506, a member of the PPE (proline-proline-glutamic acid) gene family.
引用
收藏
页码：1474 / 1477
页数：4
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