Rapid Identification and Discrimination of Brucella Isolates by Use of Real-Time PCR and High-Resolution Melt Analysis

被引：44
|
作者：
Winchell, Jonas M. [1 ]
Wolff, Bernard J. [1 ]
Tiller, Rebekah [2 ]
Bowen, Michael D. [1 ]
Hoffmaster, Alex R. [2 ]
机构：
[1] Ctr Dis Control & Prevent, Natl Ctr Immunizat & Resp Dis, Atlanta, GA 30333 USA
[2] Ctr Dis Control & Prevent, Natl Ctr Zoonot Vector Borne & Enter Dis, Atlanta, GA 30333 USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 03期
关键词：
SINGLE-NUCLEOTIDE POLYMORPHISMS; SPECIES LEVEL; MELITENSIS; DIFFERENTIATION; OVIS;
D O I：
10.1128/JCM.02021-09
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Definitive identification of Brucella species remains a challenge due to the high degree of genetic homology shared within the genus. We report the development of a molecular technique which utilizes real-time PCR followed by high-resolution melt (HRM) curve analysis to reliably type members of this genus. Using a panel of seven primer sets, we tested 153 Brucella spp. isolates with >99% accuracy compared to traditional techniques. This assay provides a useful diagnostic tool that can rapidly type Brucella isolates and has the potential to detect novel species. This approach may also prove helpful for clinical, epidemiological and veterinary investigations.
引用
收藏
页码：697 / 702
页数：6
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