Influence of lipid peroxidation on the nicotinic acetylcholine receptors in PC12 cells

被引：11
|
作者：
Guan, ZZ [1 ]
Zhang, X [1 ]
Nordberg, A [1 ]
机构：
[1] Huddinge Univ Hosp B84, Karolinska Inst, Dept Clin Neurosci Occupat Therapy & Elderly Care, Div Mol Neuropharmacol, S-14186 Huddinge, Sweden
来源：
NEUROSCIENCE LETTERS | 2000年 / 286卷 / 03期
关键词：
lipid peroxidation; iron; nicotinic acetylcholine receptor; PC12; cells;
D O I：
10.1016/S0304-3940(00)01127-7
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The expression of neuronal nicotinic acetylcholine receptors (nAChRs) influenced by lipid peroxidation has been investigated. The results showed that exposure of PC12 cells to a free radical insult (FeSO4) induced concentration-dependent increase in level of MDA (malondialdehyde), and a significant decline in MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] reduction. In cultured cells exposed to FeSO4, a significant dose-dependent decrease in [H-3] epibatidine binding sites was detected and a significant reduction in B-max value was observed in the high affinity epibatidine binding site. These results demonstrate that lipid peroxidation in cellular membranes can induce a reduction in numbers of neuronal nAChRs. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
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页码：163 / 166
页数：4
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