Improvement in microhollow production using bubble cloud cavitation by dual-frequency ultrasonic wave irradiation

被引：5

作者：

Sunaguchi, Naoki ^{[1
]}

Yamaguchi, Jun ^{[1
]}

Kanai, Takuya ^{[1
]}

Isono, Tomoaki ^{[1
]}

Yamakoshi, Yoshiki ^{[1
]}

机构：

[1] Gunma Univ, Grad Sch Engn, Kiryu, Gunma 3768515, Japan

来源：

JAPANESE JOURNAL OF APPLIED PHYSICS | 2014年 / 53卷 / 07期

关键词：

CONTRAST AGENTS; GENE-TRANSFER; IN-VITRO; SONOPORATION; TRANSFECTION; MICROBUBBLES; PLASMID; CELLS;

D O I：

10.7567/JJAP.53.07KF18

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

Bubble cloud cavitation plays an important role in sonoporation, which produces microhollows on the cell membrane using a microjet. However, it has complicated mechanisms and the optimization of the ultrasonic wave irradiation sequence is difficult. The optimization of an ultrasonic wave irradiation sequence, which allows for effective bubble cloud cavitation, is very important for producing high-quality microhollows. Thus, it is necessary to clarify the mechanism of cavitation for different-frequency ultrasonic waves. In this study, we evaluate bubble cloud cavitation for ultrasonic wave irradiations of different frequencies. The observation of bubble cloud dynamics using a high-speed camera and of microhollows using a confocal laser microscope are performed in this study. From these results, a novel ultrasonic wave irradiation sequence that uses two ultrasonic waves with different frequencies is proposed. (C) 2014 The Japan Society of Applied Physics

引用

页数：6

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