Structural characterization of the serotype O:5 O-polysaccharide antigen of the lipopolysaccharide of Escherichia coli O:5

被引：0
|
作者：
MacLean, LL [1 ]
Perry, MB [1 ]
机构：
[1] NATL RES COUNCIL CANADA,INST BIOL SCI,OTTAWA,ON K1A 0R6,CANADA
来源：
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 1997年 / 75卷 / 03期
关键词：
Escherichia coli; lipopolysaccharide; polysaccharide; O antigen; NMR;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The structure of the antigenic O-polysaccharide component of the smooth lipopolysaccharide produced by Escherichia coli serotype O:5 was investigated by composition, methylation, and periodate oxidation methods, and by 1D and 2D nuclear magnetic resonance spectroscopy. The antigenic O-chain was determined to be a high molecular weight polysaccharide composed of repeating tetrasaccharide units containing 2-acetamido-2-deoxy-D-galactose, D-galactose, D-ribose, and 3-acetamido-3,6-dideoxy-D-glucose and having the structure -[-4)-beta-D-Galp-(1-3)-alpha-D-GalpNAc-(1-4)-beta-D-Quinp3NAc-(1-3)-beta-D-Ribf-(1-]-.
引用
收藏
页码：199 / 205
页数：7
相关论文
共 50 条
  • [31] THE O1 ANTIGEN OF ESCHERICHIA-COLI - STRUCTURAL CHARACTERIZATION OF THE O1A1-SPECIFIC POLYSACCHARIDE
    JANN, B
    SHASHKOV, AS
    GUPTA, DS
    PANASENKO, SM
    JANN, K
    [J]. CARBOHYDRATE POLYMERS, 1992, 18 (01) : 51 - 57
  • [32] Characterization and biological role of the O-polysaccharide gene cluster of Yersinia enterocolitica serotype O:9
    Skurnik, Mikael
    Biedzka-Sarek, Marta
    Lubeck, Peter S.
    Blom, Tea
    Antonio Bengoechea, Jose
    Perez-Gutierrez, Camino
    Ahrens, Peter
    Hoorfar, Jeffrey
    [J]. JOURNAL OF BACTERIOLOGY, 2007, 189 (20) : 7244 - 7253
  • [33] CHARACTERIZATION OF THE O-POLYSACCHARIDE OF PASTEURELLA-HAEMOLYTICA SEROTYPE A1
    SEVERN, WB
    RICHARDS, JC
    [J]. CARBOHYDRATE RESEARCH, 1993, 240 : 277 - 285
  • [34] STRUCTURAL STUDIES OF THE ESCHERICHIA-COLI O1A O-POLYSACCHARIDE, USING THE COMPUTER-PROGRAM CASPER
    BAUMANN, H
    JANSSON, PE
    KENNE, L
    WIDMALM, G
    [J]. CARBOHYDRATE RESEARCH, 1991, 211 (01) : 183 - 190
  • [35] Structure of a teichoic acid-like O-polysaccharide of Escherichia coli O29
    Perepelov, Andrei V.
    Wang, Quan
    Senchenkova, Sof'ya N.
    Shevelev, Sergel D.
    Zhao, Guang
    Shashkov, Alexander S.
    Feng, Lu
    Knirel, Yuriy A.
    Wang, Lei
    [J]. CARBOHYDRATE RESEARCH, 2006, 341 (12) : 2176 - 2180
  • [36] Structural studies of the O-antigen polysaccharide from the enteroinvasive Escherichia coli O173
    Linnerborg, M
    Weintraub, A
    Widmalm, G
    [J]. CARBOHYDRATE RESEARCH, 1999, 320 (3-4) : 200 - 208
  • [37] STRUCTURAL STUDIES OF THE O-ANTIGEN POLYSACCHARIDES OF KLEBSIELLA O5 AND ESCHERICHIA-COLI O8
    JANSSON, PE
    LONNGREN, J
    WIDMALM, G
    LEONTEIN, K
    SLETTENGREN, K
    SVENSON, SB
    WRANGSELL, G
    DELL, A
    TILLER, PR
    [J]. CARBOHYDRATE RESEARCH, 1985, 145 (01) : 59 - 66
  • [38] STRUCTURAL STUDIES OF THE ESCHERICHIA-COLI 078 O-ANTIGEN POLYSACCHARIDE
    JANSSON, PE
    LINDBERG, B
    WIDMALM, G
    LEONTEIN, K
    [J]. CARBOHYDRATE RESEARCH, 1987, 165 (01) : 87 - 92
  • [39] Characterization of the specific O-polysaccharide structure and biosynthetic gene cluster of Yersinia pseudotuberculosis serotype O:15
    De Castro, Cristina
    Skurnik, Mikael
    Molinaro, Antonio
    Holst, Otto
    [J]. INNATE IMMUNITY, 2009, 15 (06) : 351 - 359
  • [40] Structural studies of the O-antigenic polysaccharide of the bovine mastitis isolate Escherichia coli serotype O174
    Duda, Katarzyna A.
    Fruth, Angelika
    Holst, Otto
    [J]. CARBOHYDRATE RESEARCH, 2013, 373 : 18 - 21
12345