Agrobacterium-mediated genetic transformation in Japanese persimmon

Rapid breeding progress of Japanese persimmon through conventional crossbreeding is difficult because, as with other woody plants, persimmon has a long juvenile period and large plant size. Furthermore, persimmon is a polyploid, which makes it difficult to predict segregation of characters. Recent advances in genetic transformation technology make it possible to make small specific changes in the genome of existing cultivars. Transgenic persimmon cultivars thus produced have potential for commercial success and grower acceptance because the unique genetic constitution of the cultivars has not been disturbed. To apply the technology to persimmon breeding, we have developed a reliable and efficient protocol for Agrobacterium-mediated transformation of persimmon and integrated several genes of horticultural importance to the persimmon genome. In this review article, the current status of our study on genetic manipulation of persimmon, including the genetic engineering of 1) resistance to pests, 2) resistance to disease, and 3) tolerance to salt stress, is summarized.