Detection of Trypanosoma cruzi and T.rangeli Infections from Rhodnius pallescens Bugs by Loop-Mediated Isothermal Amplification (LAMP)

被引：27
|
作者：
Thekisoe, Oriel M. M. [1 ]
Rodriguez, Carol V. [2 ]
Rivas, Francisco [2 ]
Coronel-Servian, Andrea M. [1 ]
Fukumoto, Shinya [1 ]
Sugimoto, Chihiro [3 ]
Kawazu, Shin-Ichiro [1 ]
Inoue, Noboru [1 ]
机构：
[1] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan
[2] Univ Panama, Coll Med, Dept Microbiol, Panama City, Panama
[3] Hokkaido Univ, Res Ctr Zoonosis Control, Sapporo, Hokkaido, Japan
来源：
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2010年 / 82卷 / 05期
关键词：
CHAIN-REACTION AMPLIFICATION; RAPID DETECTION; RANGELI; BLOOD; DNA; AREA; GENE;
D O I：
10.4269/ajtmh.2010.09-0533
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
We have developed two loop-mediated isothermal amplification (LAMP) assays for specific detection of Trypanosoma cruzi and Trypanosoma rangeli based on the 18S ribosomal RNA (rRNA) and the small nucleolar RNA (snoRNA) genes, respectively. The detection limit of the assays is 100 fg and 1 pg for T cruzi and T rongeli, respectively. with reactions conducted in 60 minutes. The two LAMP assays were used in detection of T cruzi and T rangell infections in comparison with polymerase chain reaction (PCR) for DNA samples extracted from Rhodnius pallescens hugs collected from palm trees in Panama. Out of a total of 52 DNA samples from R. pallescens bugs 17 (33%) and 14 (27%) were T cruzi-positive by LAMP and PCR, respectively, while, 7 (13%) and 4(8%) were T.rangeli-positive by LAMP and PCR, respectively. Further evaluation of these LAMP assays is needed, especially with specimens collected from human patients as well as blood kept for transfusion purposes.
引用
收藏
页码：855 / 860
页数：6
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