Development and applications of enhanced green fluorescent protein mutants

被引：150
|
作者：
Stauber, RH [1 ]
Horie, K [1 ]
Carney, P [1 ]
Hudson, EA [1 ]
Tarasova, NI [1 ]
Gaitanaris, GA [1 ]
Pavlakis, GN [1 ]
机构：
[1] NCI, ABL Basic Res Program, FCRDC, Frederick, MD 21702 USA
来源：
BIOTECHNIQUES | 1998年 / 24卷 / 03期
关键词：
D O I：
10.2144/98243rr01
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) Florescent protein, gave 50-fold-100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon expression in mammalian cells. GFPsg25 and BFPsg50 have different excitation and emission maxima. This allows their use as an efficient dual-color tagging system and their independent detection in living cells.
引用
收藏
页码：462 / +
页数：8
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