DEVELOPMENT OF A LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF SIX BENZODIAZEPINES IN HUMAN PLASMA AFTER SOLID-PHASE EXTRACTION

被引：17

作者：

Fernandez, Purificacion ^{[1
]}

Vazquez, Catherine ^{[1
]}

Lorenzo, Rosa A. ^{[2
]}

Carro, Antonia M. ^{[2
]}

Bermejo, Ana M. ^{[1
]}

机构：

[1] Fac Med, Forens Toxicol Serv, Inst Legal Med, Santiago De Compostela 15782, Spain

[2] Fac Chem, Dept Analyt Chem, Santiago De Compostela, Spain

来源：

ANALYTICAL LETTERS | 2010年 / 43卷 / 06期

关键词：

Benzodiazepines; HPLC-DAD; Plasma; Solid-phase extraction; PHOTODIODE-ARRAY DETECTION; MASS-SPECTROMETRY; WHOLE-BLOOD; LC-MS/MS; GAS-CHROMATOGRAPHY; BIOLOGICAL-FLUIDS; HPLC METHOD; ORAL FLUID; GC-MS; METABOLITES;

D O I：

10.1080/00032710903492490

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and fast procedure is presented for the simultaneous determination of alprazolam, bromazepam, diazepam, lorazepam, lormetazepam, and tetrazepam in human plasma. The drugs were isolated by solid-phase extraction, and the subsequent analysis was carried out by high-performance liquid chromatography coupled with a diode-array detector (HPLC-DAD). This allowed recoveries of around 90% to be obtained with good sensitivity, selectivity, and resolution of the eluting peaks. The detector response at 230 nm was linear within the benzodiazepine concentration range of 20-4000 ng/mL in human plasma, with values of less than 6.7% and 5.4% for precision and accuracy, respectively.

引用

页码：1075 / 1084

页数：10

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