DEVELOPMENT OF A LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF SIX BENZODIAZEPINES IN HUMAN PLASMA AFTER SOLID-PHASE EXTRACTION

被引：17
|
作者：
Fernandez, Purificacion [1 ]
Vazquez, Catherine [1 ]
Lorenzo, Rosa A. [2 ]
Carro, Antonia M. [2 ]
Bermejo, Ana M. [1 ]
机构：
[1] Fac Med, Forens Toxicol Serv, Inst Legal Med, Santiago De Compostela 15782, Spain
[2] Fac Chem, Dept Analyt Chem, Santiago De Compostela, Spain
来源：
ANALYTICAL LETTERS | 2010年 / 43卷 / 06期
关键词：
Benzodiazepines; HPLC-DAD; Plasma; Solid-phase extraction; PHOTODIODE-ARRAY DETECTION; MASS-SPECTROMETRY; WHOLE-BLOOD; LC-MS/MS; GAS-CHROMATOGRAPHY; BIOLOGICAL-FLUIDS; HPLC METHOD; ORAL FLUID; GC-MS; METABOLITES;
D O I：
10.1080/00032710903492490
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A simple and fast procedure is presented for the simultaneous determination of alprazolam, bromazepam, diazepam, lorazepam, lormetazepam, and tetrazepam in human plasma. The drugs were isolated by solid-phase extraction, and the subsequent analysis was carried out by high-performance liquid chromatography coupled with a diode-array detector (HPLC-DAD). This allowed recoveries of around 90% to be obtained with good sensitivity, selectivity, and resolution of the eluting peaks. The detector response at 230 nm was linear within the benzodiazepine concentration range of 20-4000 ng/mL in human plasma, with values of less than 6.7% and 5.4% for precision and accuracy, respectively.
引用
收藏
页码：1075 / 1084
页数：10
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