Backbone and side-chain assignments of an effector membrane localization domain from Vibrio vulnificus MARTX toxin

被引：3

作者：

Brothers, Michael C. ^{[1
]}

Geissler, Brett ^{[2
]}

Hisao, Grant S. ^{[1
]}

Wilson, Brenda A. ^{[3
]}

Satchell, Karla J. F. ^{[2
]}

Rienstra, Chad M. ^{[1
,4
,5
]}

机构：

[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

[2] Northwestern Univ, Dept Microbiol Immunol, Chicago, IL 60611 USA

[3] Univ Illinois, Dept Microbiol, Urbana, IL 61801 USA

[4] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA

[5] Univ Illinois, Ctr Biophys & Computat Biol, Urbana, IL 61801 USA

来源：

BIOMOLECULAR NMR ASSIGNMENTS | 2014年 / 8卷 / 02期

关键词：

Membrane localization domain; Four helical bundle; MARTX toxins; Pasteurella multocida toxin; LARGER PROTEINS; SPECTROSCOPY; RESONANCES; SPECTRA; AMIDE;

D O I：

10.1007/s12104-013-9488-0

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

H-1, C-13, and N-15 chemical shift assignments are presented for the isolated four-helical bundle membrane localization domain from the domain of unknown function 5 (DUF5) effector (MLDVvDUF5) of the MARTX toxin from Vibrio vulnificus in its solution state. We have assigned 97 % of all backbone and side-chain carbon atoms, including 96 % of all backbone residues. Secondary chemical shift analysis using TALOS+ demonstrates four helices that align with those predicted by structure homology modeling using the MLDs of Pasteurella multocida toxin (PMT) and the clostridial TcdB and TcsL toxins as templates. Future studies will be towards solving the structure and determining the dynamics in the solution state.

引用

页码：225 / 228

页数：4

共 50 条

[21] Backbone and side-chain assignments for a novel CBM69 starch binding domain AmyP-SBD
Li, Xinxin
Yu, Jigang
Zhang, Jiahai
Sun, Hongbin
Zhang, Xuecheng
BIOMOLECULAR NMR ASSIGNMENTS, 2017, 11 (02) : 235 - 237
[22] Backbone and side-chain assignments for a novel CBM69 starch binding domain AmyP-SBD
Xinxin Li
Jigang Yu
Jiahai Zhang
Hongbin Sun
Xuecheng Zhang
Biomolecular NMR Assignments, 2017, 11 : 235 - 237
[23] THE BACKBONE AND SIDE-CHAIN CONFORMATIONS OF THE CYCLIC TETRAPEPTIDE HC-TOXIN
MASCAGNI, P
POPE, M
GIBBONS, WA
CIUFFETTI, LM
KNOCHE, HW
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1983, 113 (01) : 10 - 17
[24] SIDE-CHAIN AND BACKBONE ASSIGNMENTS IN ISOTOPICALLY LABELED PROTEINS FROM 2 HETERONUCLEAR TRIPLE RESONANCE EXPERIMENTS
LOGAN, TM
OLEJNICZAK, ET
XU, RX
FESIK, SW
FEBS LETTERS, 1992, 314 (03): : 413 - 418
[25] Backbone and side-chain resonance assignments for the tmRNA-binding protein, SmpB, from Mycobacterium tuberculosis
Juanjuan Yang
Yindi Liu
Zhao Liu
Chun Meng
Donghai Lin
Biomolecular NMR Assignments, 2017, 11 : 175 - 179
[26] Backbone and ILV side-chain NMR resonance assignments of the catalytic domain of human deubiquitinating enzyme USP7
Gabrielle Valles
Alexandra Pozhidaeva
Dmitry M. Korzhnev
Irina Bezsonova
Biomolecular NMR Assignments, 2022, 16 : 197 - 203
[27] Backbone and side-chain resonance assignments for the tmRNA-binding protein, SmpB, from Mycobacterium tuberculosis
Yang, Juanjuan
Liu, Yindi
Liu, Zhao
Meng, Chun
Lin, Donghai
BIOMOLECULAR NMR ASSIGNMENTS, 2017, 11 (02) : 175 - 179
[28] Backbone and ILV side-chain NMR resonance assignments of the catalytic domain of human deubiquitinating enzyme USP7
Valles, Gabrielle
Pozhidaeva, Alexandra
Korzhnev, Dmitry M.
Bezsonova, Irina
BIOMOLECULAR NMR ASSIGNMENTS, 2022, 16 (02) : 197 - 203
[29] Backbone and side-chain resonance assignments of centromeric protein Scm3 from Saccharomyces cerevisiae
Bhattacharya, Anusri
Shukla, Vaibhav Kumar
Hosur, Ramakrishna V.
Kumar, Ashutosh
BIOMOLECULAR NMR ASSIGNMENTS, 2019, 13 (02) : 267 - 273
[30] Backbone and side-chain resonance assignments of centromeric protein Scm3 from Saccharomyces cerevisiae
Anusri Bhattacharya
Vaibhav Kumar Shukla
Ramakrishna V. Hosur
Ashutosh Kumar
Biomolecular NMR Assignments, 2019, 13 : 267 - 273

← 1 2 3 4 5 →