Genetic analysis of variation in micropropagated plants of Melia azedarach L.

被引:10
|
作者
Olmos, SE
Lavia, G
Di Renz, M
Mroginsk, L
Echenique, V
机构
[1] Univ Nacl Sur, CONICET, RA-8000 Bahia Blanca, Buenos Aires, Argentina
[2] Univ Nacl Sur, Dept Agron, RA-8000 Bahia Blanca, Buenos Aires, Argentina
[3] Univ Nacl Nordeste, Fac Ciencias Agrarias, RA-3400 Corrientes, Argentina
[4] Univ Nacl Rio Cuarto, Fac Agron & Vet, RA-5800 Rio Cuarto, Argentina
关键词
somaclonal variation; RAPD; diversity analysis; chromosome counting;
D O I
10.1079/IVP2002319
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Plants were regenerated by shoot multiplication from four clones of Melia azedarach L. during 12 mo. of subculturing. One hundred and one of these plants were examined by randomly amplified polymorphic DNA analysis. All regenerated plants showed at least one polymorphism. However, no chromosome number alterations were observed. The pattern of variation obtained by principal coordinated analysis showed a random distribution of variation among regenerated plants and their controls, indicating that genetic alterations were not cumulative during in vitro culture. Similar results were found using Shannon's index, which revealed that 50% of the observed diversity resided among plants coming from the same subculture generation. This high intraclonal variation does not provide a clear scenario for predicting the amount of culture time required to preserve genetic fidelity in commercially micropropagated M. azedarach plants. Our work suggests that other mechanisms, such as chimerism, contribute to intraclonal heterogeneity in vitro.
引用
收藏
页码:617 / 622
页数:6
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