Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles

被引:1146
|
作者
Li, HX [1 ]
Rothberg, L [1 ]
机构
[1] Univ Rochester, Dept Chem, Rochester, NY 14627 USA
关键词
D O I
10.1073/pnas.0406115101
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We find that single- and double-stranded oligonucleotides have different propensities to adsorb on gold nanoparticles in colloidal solution. We use this observation to design a hybridization assay based on color changes associated with gold aggregation. Because the underlying adsorption mechanism is electrostatic, no covalent functionalization of the gold, the probe, or the target DNA is required. Hybridization conditions can be optimized because it is completely separated from the detection step. The assay is complete within 5 min, and < 100 femtomoles of target produces color changes observable without instrumentation. Single-base-pair mismatches are easily detected.
引用
收藏
页码:14036 / 14039
页数:4
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