Cytosine-bulge-dependent fluorescence quenching for the real-time hairpin primer PCR

被引：8
|
作者：
Takei, F. [1 ]
Chen, X. [1 ]
Yu, G. [1 ]
Shibata, T. [1 ]
Dohno, C. [1 ]
Nakatani, K. [1 ]
机构：
[1] Osaka Univ, Inst Sci & Ind Res, Ibaraki 5670047, Japan
来源：
CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 96期
基金：
日本科学技术振兴机构;
关键词：
SINGLE-NUCLEOTIDE-POLYMORPHISM; POLYMERASE-CHAIN-REACTION; ALLELE-SPECIFIC PCR; MOLECULAR BEACONS; FIT-PROBES; HYBRIDIZATION PROBES; SECONDARY-STRUCTURE; RT-PCR; DNA; RNA;
D O I：
10.1039/c4cc06780k
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The progress of a polymerase chain reaction (PCR) was sensitively monitored based on the increase in fluorescence of N, N'-bis(3-aminopropyl)- 2,7-diamino-1,8-naphthyridine, which was covalently anchored on the cytosine bulge directly neighbouring the 5'-T_G-3'/5'-CCA-3' sequence in the hairpin tag at the 5' end of the PCR primer.
引用
收藏
页码：15195 / 15198
页数：4
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