Determination of polymyxin E1 in rat plasma by high-performance liquid chromatography

被引：26
|
作者：
Gmur, DJ [1 ]
Bredl, CR [1 ]
Steele, SJ [1 ]
Cai, SP [1 ]
VanDevanter, DR [1 ]
Nardella, PA [1 ]
机构：
[1] Chiron Corp, Seattle, WA 98119 USA
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 789卷 / 02期
关键词：
polymyxin E-1;
D O I：
10.1016/S1570-0232(03)00162-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A precise and accurate HPLC assay for polymyxin E-1 in rat and dog plasma has been validated. Samples and standards are extracted from plasma with a 96-well C-8 extraction disk plate. Sample extracts are derivatized with dansyl chloride, and polymyxin E-1 derivative is quantitated on a C-8 column by HPLC with fluorescence detection. The assay is linear in the range of 0.050-5.00 mug/ml for polyrnyxim E-1. The precision and accuracy of polymyxin E-1 plasma assay was well within the recommended limits set in the FDA Guidance for Bioanalytical Method Validation. Polymyxin E-1 stability in rat and dog plasma for 24 h at room temperature and through three freeze-thaw cycles was demonstrated. (C) 2003 Elsevier Science B.V. All rights reserved.
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页码：365 / 372
页数：8
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