Application of Green Silver Nanoparticles Synthesized using Leaf Extract of Tridax procumbens for Preparation of Clinical Antimicrobial Bandages

Objectives: To green synthesize silver nanoparticles by using weed plant Tridax procumbens also prepare and check antimicrobial activity of silver nanoparticles impregnated bandages against one gram positive bacteria and one-gram negative. Methods: The green synthesis of silver nanoparticles was done by chemical precipitation method using Tridax leaves extract as reducing agent. Synthesis of silver nanoparticles are characterized by UV-Visible spectroscopy, Dynamic light scattering (DLS), Zeta potential analysis, Fourier transform infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and X-ray diffraction analysis (XRD) to confirm size distribution, composition and crystalline nature of silver nanoparticles. Results: The peak was shown at 446nm by UV-Vis spectrophotometer and Scanning Electron Microscopy analysis reveals the spherical-shaped nanoparticles with a mean size of 99nm. Fourier transform infrared spectroscopy proved that the phytochemicals in extract were responsible for formation of the silver nanoparticles and acted as reducing and stabilizing agent. XRD study have revealed the highly crystalline nature of nanoparticles. These nanoparticles were impregnated onto bandages and they showed appreciable inhibitory activity against Escherichia coli (6mm) and Staphylococcus aureus (2mm). Conclusion: The characterization results shown the characteristic nature of the green synthesized silver nanoparticles. Thus the green synthesis was ecofriendly and nanoparticles showed promising antimicrobial activity. These antibacterial bandages can potentially be used for treating and open and infection-prone wounds.