Membrane protein reconstitution for functional and structural studies

被引：23
|
作者：
Wang LiGuo [1 ]
Tonggu LiGe [1 ]
机构：
[1] Univ Washington, Dept Biol Struct, Seattle, WA 98195 USA
来源：
SCIENCE CHINA-LIFE SCIENCES | 2015年 / 58卷 / 01期
基金：
美国国家卫生研究院;
关键词：
membrane protein; reconstitution; Ca2+ signaling; Ca2+ mediators; Ca2+-regulated enzymes; Ca2+ transducers; SINGLE-CHANNEL FUNCTION; ACETYLCHOLINE-RECEPTOR; VESICLE FORMATION; LIPID-MEMBRANE; DETERGENT; PROTEOLIPOSOMES; PURIFICATION; LIPOSOMES; PHOSPHORYLATION; PHOSPHOLIPIDS;
D O I：
10.1007/s11427-014-4769-0
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Membrane proteins are involved in various critical biological processes, and studying membrane proteins represents a major challenge in protein biochemistry. As shown by both structural and functional studies, the membrane environment plays an essential role for membrane proteins. In vitro studies are reliant on the successful reconstitution of membrane proteins. This review describes the interaction between detergents and lipids that aids the understanding of the reconstitution processes. Then the techniques of detergent removal and a few useful techniques to refine the formed proteoliposomes are reviewed. Finally the applications of reconstitution techniques to study membrane proteins involved in Ca2+ signaling are summarized.
引用
收藏
页码：66 / 74
页数：9
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