Papain Adsorption on Chitosan-Coated Nylon-Based Immobilized Metal Ion (Cu2+, Ni2+, Zn2+, Co2+) Affinity Membranes

被引：8

作者：

He, Zhi-Yan ^{[1
]}

Christopher, Branford-White ^{[2
]}

Zhou, Yu-Ting ^{[1
]}

Nie, Hua-Li ^{[1
]}

Zhu, Li-Min ^{[1
]}

机构：

[1] Donghua Univ, Coll Chem Chem Engn & Biotechnol, Shanghai 201620, Peoples R China

[2] London Metropolitan Univ, Inst Hlth Res & Policy, London, England

来源：

SEPARATION SCIENCE AND TECHNOLOGY | 2010年 / 45卷 / 04期

关键词：

adsorption; affinity membrane; metal ion; papain; PURIFICATION; CELLULOSE; CHROMATOGRAPHY; PROTEIN; SEPARATION; KINETICS; EXCHANGE; REMOVAL;

D O I：

10.1080/01496390903484784

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel immobilized metal affinity membrane was prepared for papain adsorption in this article. Higher papain adsorption capacity between 43-67mg/g was observed and the adsorption isotherm fitted the Freundlich equation. Experimental data were analyzed using two adsorption kinetic models. The pseudo-second-order kinetic model provided better correlation to the experimental results. A significant amount of the adsorbed papain was eluted by 1.0M NaSCN at pH 5.0 for all affinity membranes. It was concluded that the novel chitosan-coated nylon-based immobilized metal ion affinity membrane could be applied for the large-scale isolation of papain without resulting in enzyme denaturation.

引用

页码：525 / 534

页数：10

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