Papain Adsorption on Chitosan-Coated Nylon-Based Immobilized Metal Ion (Cu2+, Ni2+, Zn2+, Co2+) Affinity Membranes

被引：8
|
作者：
He, Zhi-Yan [1 ]
Christopher, Branford-White [2 ]
Zhou, Yu-Ting [1 ]
Nie, Hua-Li [1 ]
Zhu, Li-Min [1 ]
机构：
[1] Donghua Univ, Coll Chem Chem Engn & Biotechnol, Shanghai 201620, Peoples R China
[2] London Metropolitan Univ, Inst Hlth Res & Policy, London, England
来源：
SEPARATION SCIENCE AND TECHNOLOGY | 2010年 / 45卷 / 04期
关键词：
adsorption; affinity membrane; metal ion; papain; PURIFICATION; CELLULOSE; CHROMATOGRAPHY; PROTEIN; SEPARATION; KINETICS; EXCHANGE; REMOVAL;
D O I：
10.1080/01496390903484784
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel immobilized metal affinity membrane was prepared for papain adsorption in this article. Higher papain adsorption capacity between 43-67mg/g was observed and the adsorption isotherm fitted the Freundlich equation. Experimental data were analyzed using two adsorption kinetic models. The pseudo-second-order kinetic model provided better correlation to the experimental results. A significant amount of the adsorbed papain was eluted by 1.0M NaSCN at pH 5.0 for all affinity membranes. It was concluded that the novel chitosan-coated nylon-based immobilized metal ion affinity membrane could be applied for the large-scale isolation of papain without resulting in enzyme denaturation.
引用
收藏
页码：525 / 534
页数：10
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