Recombinant polypeptide antigen-based immunoglobulin G enzyme-linked immunosorbent assay for serodiagnosis of dengue

被引：6
|
作者：
Dos Santos, Flavia B.
Nogeira, Rita Maria R.
Lima, Monique R. Q.
De Simone, Thatiane S.
Schatzmayr, Hermann G.
Lemes, Elezer A. B.
Harris, Eva
Miagostovich, Marize P.
机构：
[1] Fiocruz MS, Inst Oswaldo Cruz, Flavivirus Lab, Dept Virol, BR-21045360 Rio De Janeiro, Brazil
[2] Dept Technol Dev, BR-21045360 Rio De Janeiro, Brazil
[3] Univ Calif Berkeley, Sch Publ Hlth, Div Infect Dis, Berkeley, CA 94720 USA
来源：
CLINICAL AND VACCINE IMMUNOLOGY | 2007年 / 14卷 / 05期
关键词：
D O I：
10.1128/CVI.00474-06
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
We have developed an indirect enzyme-linked immunosorbent assay for detection of anti-dengue virus (DENV) immunoglobulin G antibodies using four recombinant DENV envelope polypeptides as antigens, which demonstrated a sensitivity of 89.4% and a specificity of 93.3%. These easily produced antigens are a feasible, cost-effective alternative for generating reagents for dengue serological tests.
引用
收藏
页码：641 / 643
页数：3
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