Protein extraction by calix[6]arene in an aliphatic organic solvent

被引：0
|
作者：
Shimojo, K [1 ]
Watanabe, J
Oshima, T
Goto, M
机构：
[1] Kyushu Univ, Grad Sch Engn, Dept Appl Chem, Fukuoka 8128581, Japan
[2] Saga Univ, Fac Engn, Dept Ind Chem, Saga 8408502, Japan
来源：
SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN | 2003年 / 10卷
关键词：
D O I：
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中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Protein extraction was performed by a calix[6]arene carboxylic acid derivative in isooctane. Although the calixarene has a drawback in that it is not solubilized in aliphatic organic solvents, addition of alcohols overcomes the problem. The effect of alcohols on the extraction of cytochrome c (Cyt-c) was investigated with calix[6]arene in isooctane. Normal octanol provided the best result for the Cyt-c extraction. The pH value in the aqueous phase was one of the key operational factors in the liquid-liquid extraction process. It was confirmed that the extracted proteins maintain their biological activity during the extraction operation.
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页码：185 / 189
页数：5
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