Preparation, characterization, and in vivo evaluation of Rose damascene extract loaded solid lipid nanoparticles for targeted brain delivery

According to a variety of experiments, Rose damascene may lead to memory enhancement and acetylcholine esterase inhibition. However, Rose damascene cannot pass through the blood-brain barrier due to its hydrophilic contents. Solid lipid nanoparticles (SLNs) are suitable carriers for brain drug delivery. Herein, SLNs were made by micro-emulsion method. Then, lactoferrin was covalently attached to the surface of the nanoparticles by amide bond interaction for targeted delivery. The nanoparticle properties and the amount of attached lactoferrin were calculated. The effect of the selected compounds on scopolamine-induced animals was also measured by Y-maze, passive avoidance test, elevated plus maze, and forced swim test. The results revealed that the size and zeta potential of nanoparticles were 52 nm and - 13 mV before conjugation, and 161 nm and - 16 mV after conjugation, respectively. The percentage of entrapment efficiency and drug loading before conjugation was 98 ,93.6 and, after conjugation, was 11.2, 15.9, respectively. According to Y-maze and passive avoidance test results, Rose damascene can enhance short-term memory and may also reduce anxiety and depression in scopolamine-induced animals.