Effects of overexpressing p14ARF on the proliferation in human melanoma cells

被引：0
|
作者：
Peng, LX [1 ]
Zhang, W [1 ]
Liu, HT [1 ]
He, DC [1 ]
Gao, P [1 ]
机构：
[1] Beijing Normal Univ, Coll Life Sci, Minist Educ, Key Lab Cell Proliferat & Regulat Biol, Beijing 100875, Peoples R China
来源：
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 2004年 / 31卷 / 02期
关键词：
p14(ARF); p53; ERK; cell cycle arrest;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
ARF can induce cell cycle arrest or apoptosis. By using the A375 cell overexpressing p14(ARF) as a model, the molecular machanism of cell cycle arrest by ARF was studied. It was indicated that overexpressing p14(ARF) resulted in G1-phase and G2-phase arrest. In the A375 cells overexpressing p14(ARF), the level of p53, p21(cipl) and p27(kipl) was upregulated, but the protein lever of p-ERK1/2, Cyclin D1 and Cyclin E was inhibited. The results indicated that p14(ARF) induced the cell cycle arrest of A375 not only in a p53-dependent way, but also in coopration with the ERK signal transduction pathway.
引用
收藏
页码：177 / 180
页数：4
相关论文
共 13 条
  • [1] ERK-1/2 and p38 kinase oppositely regulate nitric oxide-induced apoptosis of chondrocytes in association with p53, caspase-3, and differentiation status
    Kim, SJ
    Ju, JW
    Oh, CD
    Yoon, YM
    Song, WK
    Kim, JH
    Yoo, YJ
    Bang, OS
    Kang, SS
    Chun, JS
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (02) : 1332 - 1339
  • [2] Cyclin D1 expression is regulated positively by the p42/p44(MAPK) and negatively by the p38/HOG(MAPK) pathway
    Lavoie, JN
    LAllemain, G
    Brunet, A
    Muller, R
    Pouyssegur, J
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (34) : 20608 - 20616
  • [3] Functional inactivation of the retinoblastoma protein requires sequential modification by at least two distinct cyclin-cdk complexes
    Lundberg, AS
    Weinberg, RA
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (02) : 753 - 761
  • [4] Quercetin exerts multiple inhibitory effects on vascular smooth muscle cells: role of ERK1/2, cell-cycle regulation, and matrix metalloproteinase-9
    Moon, SK
    Cho, GO
    Jung, SY
    Gal, SW
    Kwon, TK
    Lee, YC
    Madamanchi, NR
    Kim, CH
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 301 (04) : 1069 - 1078
  • [5] IDENTIFICATION OF THE ACTIVE-REGION OF THE DNA-SYNTHESIS INHIBITORY GENE P21(SDI1/CIP1/WAF1)
    NAKANISHI, M
    ROBETORYE, RS
    ADAMI, GR
    PEREIRASMITH, OM
    SMITH, JR
    [J]. EMBO JOURNAL, 1995, 14 (03): : 555 - 563
  • [6] Mitogen-activated protein kinases contribute to reactive oxygen species-induced cell death in renal proximal tubule epithelial cells
    Ramachandiran, S
    Huang, QH
    Dong, J
    Lau, SS
    Monks, TJ
    [J]. CHEMICAL RESEARCH IN TOXICOLOGY, 2002, 15 (12) : 1635 - 1642
  • [7] Sambrook J, 1989, MOL CLONING LAB MANU, P870
  • [8] The INK4α/ARF locus in murine tumorigenesis
    Serrano, M
    [J]. CARCINOGENESIS, 2000, 21 (05) : 865 - 869
  • [9] D-TYPE CYCLINS
    SHERR, CJ
    [J]. TRENDS IN BIOCHEMICAL SCIENCES, 1995, 20 (05) : 187 - 190
  • [10] The ARF/p53 pathway
    Sherr, CJ
    Weber, JD
    [J]. CURRENT OPINION IN GENETICS & DEVELOPMENT, 2000, 10 (01) : 94 - 99
12