J774 Macrophage-like Cell Line Cytokine and Chemokine Patterns Are Modulated by Francisella tularensis LVS Strain Infection

被引：1
|
作者：
Holicka, M.
Novosad, J.
Kudlova, M.
Loudova, M.
Andrys, C.
Krejsek, J. [1 ]
机构：
[1] Charles Univ Prague, Univ Hosp, Dept Clin Immunol & Allergol, Hradec Kralove 50005, Czech Republic
来源：
FOLIA MICROBIOLOGICA | 2010年 / 55卷 / 02期
关键词：
NECROSIS-FACTOR-ALPHA; NITRIC-OXIDE; INTERFERON-GAMMA; MYCOBACTERIUM-MICROTI; TNF-ALPHA; EXPRESSION; ACTIVATION; INTERLEUKIN-12; SECRETION; BLOOD;
D O I：
10.1007/s12223-010-0028-3
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Mutual interactions were investigated between intracellular parasitic bacterium Francisella tularensis (F.t.; highly virulent bacterium responsible for tularemia, replicating within the host macrophages) and murine macrophage-like cell line J774. Recombinant murine lymphokine INF-gamma and/or LPS derived from E. coli were determined to stimulate in vitro antimicrobial activity of macrophage-like J774 cell line against the live vaccine strain (LVS) of F.t. through their ability to produce proinflammatory cytokines and chemokines. F.t. infection up-regulated IL-12 p40 production and down-regulated TNF-alpha production by stimulated macrophages; on the other hand, F.t. infection did not affect the production of IL-8, IL-6, MCP-5, and RANTES by stimulated macrophages. This showed that F.t. infection modulates the cytokine synthesis by J774 macrophage cell line.
引用
收藏
页码：191 / 200
页数：10
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