A Rapid Screening Workflow to Identify Potential Combination Therapy for GBM using Patient-Derived Glioma Stem Cells

被引：0
|
作者：
Hu, Ziyi [1 ]
Zhou, Tingting [1 ]
Wu, Fangrong [1 ]
Lin, Fan [1 ,2 ,3 ,4 ,5 ]
机构：
[1] Nanjing Med Univ, Sch Basic Med Sci, Dept Cell Biol, Nanjing, Peoples R China
[2] Nanjing Med Univ, Inst Brain Tumors, Nanjing, Peoples R China
[3] Nanjing Med Univ, Key Lab Rare Metab Dis, Nanjing, Peoples R China
[4] Nanjing Med Univ, Affiliated Canc Hosp, Nanjing, Peoples R China
[5] Key Lab Human Funct Genom Jiangsu Prov, Nanjing, Peoples R China
来源：
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2021年 / 169期
基金：
中国国家自然科学基金;
关键词：
D O I：
10.3791/62312
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The glioma stem cells (GSCs) are a small fraction of cancer cells which play essential roles in tumor initiation, angiogenesis, and drug resistance in glioblastoma (GBM), the most prevalent and devastating primary brain tumor. The presence of GSCs makes the GBM very refractory to most of individual targeted agents, so high-throughput screening methods are required to identify potential effective combination therapeutics. The protocol describes a simple workflow to enable rapid screening for potential combination therapy with synergistic interaction. The general steps of this workflow consist of establishing luciferase-tagged GSCs, preparing matrigel coated plates, combination drug screening, analyzing, and validating the results.
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页数：10
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