Optimizing the fluorescent yield in two-photon laser scanning microscopy with dispersion compensation

被引：27
|
作者：
Field, Jeffrey J. [1 ,2 ]
Carriles, Ramon [1 ,2 ]
Sheetz, Kraig E. [1 ,2 ]
Chandler, Eric V. [1 ,2 ]
Hoover, Erich E. [1 ,2 ]
Tillo, Shane E. [3 ]
Hughes, Thom E. [3 ]
Sylvester, Anne W. [4 ]
Kleinfeld, David [5 ,6 ]
Squier, Jeff A. [1 ,2 ]
机构：
[1] Colorado Sch Mines, Ctr Microintegrated Opt Adv Bioimaging & Control, Golden, CO 80401 USA
[2] Colorado Sch Mines, Dept Phys, Golden, CO 80401 USA
[3] Montana State Univ, Dept Cell Biol & Neurosci, Bozeman, MT 59717 USA
[4] Univ Wyoming, Dept Mol Biol, Laramie, WY 82071 USA
[5] Univ Calif San Diego, Dept Phys, Grad Program Neurosci, La Jolla, CA 92093 USA
[6] Univ Calif San Diego, Ctr Neural Circuits & Behav, La Jolla, CA 92093 USA
来源：
OPTICS EXPRESS | 2010年 / 18卷 / 13期
基金：
美国国家科学基金会;
关键词：
ULTRASHORT PULSES; MULTIPHOTON; EFFICIENCY; PROTEIN; SIGNAL;
D O I：
10.1364/OE.18.013661
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
A challenge for nonlinear imaging in living tissue is to maximize the total fluorescent yield from each fluorophore. We investigated the emission rates of three fluorophores-rhodamine B, a red fluorescent protein, and CdSe quantum dots-while manipulating the phase of the laser excitation pulse at the focus. In all cases a transform-limited pulse maximized the total yield to insure the highest signal-to-noise ratio. Further, we find evidence of fluorescence antibleaching in quantum dot samples. (C) 2010 Optical Society of America
引用
收藏
页码：13661 / 13672
页数：12
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