A comparison between the distinct inward currents activated in rat cultured dorsal root ganglion neurones by intracellular flash photolysis of two forms of caged cyclic guanosine monophosphate

被引：8

作者：

Pollock, J

Crawford, JH

Wootton, JF

Corrie, JET

Scott, RH ^{[1
]}

机构：

[1] Univ Aberdeen, Inst Med Sci, Inst Biomed Sci, Aberdeen AB25 2ZD, Scotland

[2] Cornell Univ, Coll Vet Med, Dept Biomed Sci, Ithaca, NY 14853 USA

[3] Natl Inst Med Res, London NW7 1AA, England

来源：

NEUROSCIENCE LETTERS | 2003年 / 338卷 / 02期

关键词：

cyclic guanosine monophosphate; ryanodine; calcium mobilisation; sensory neurone; cyclic nucleotide-gated channel; caged compounds; cyclic ADPribose;

D O I：

10.1016/S0304-3940(02)01393-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Whole cell inward currents activated by intracellular photorelease of cyclic guanosine monophosphate (cGMP) were investigated in cultured dorsal root ganglion (DRG) neurones. The actions of two distinct types of caged cGMP (NPE-caged cGMP and a highly water-soluble caged cGMP) were compared. Rapidly activating inward currents were evoked by cGMP in a subpopulation (12.5%) of neurones and these currents may be due to activity of cyclic nucleotide-gated channels. In contrast in 52% of DRG neurones intracellular photorelease of cGMP activated a delayed Ca2+-dependent inward current through the generation of cyclic ADPribose and mobilisation of Ca2+ from ryanodine sensitive intracellular stores. Similar delayed inward currents were activated by both caged compounds but only NPE-caged cGMP evoked rapidly activating currents. Cyclic GMP appears to increase excitability in some DRG neurones by diverse mechanisms. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：143 / 146

页数：4